BUSINESS
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In vitro test system
- In vitro test
- Animal Replacement Test
In vitro effectiveness evaluation test
We provide in vitro or cell testing services to evaluate the toxicity and effectiveness of test substances for product development or research purposes in various fields such as pharmaceuticals, cosmetics, and health functional foods.
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Cytotoxicity test
This is a test to determine the concentration of a sample that is not toxic to cells in vitro, identifying the maximum concentration that does not affect cell viability when cells are treated with serially diluted samples and cultured for a certain period of time.
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- Sample type: liquid, powder, and solid
- Methods : MTT, WST-8, LDH assay
- Cell line: MRC-5, Hatcat, decision to be made after consultation if other cells are desired
- Time required: 3-4 weeks
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Anticancer test
This test confirms that the proliferation of cancer cells is inhibited or Dead, and cell viability can be identified in various cancer cells and resistant cancer cells.
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- Sample type: liquid, powder, and solid
- Methods : MTT, WST-8. NRU, Colony assay 등
- Cell line: ovarian cancer, cervical cancer, breast cancer, blood cancer, lung cancer, colon cancer, stomach cancer, liver cancer, pancreatic cancer cell, and drug-resistant cell line, decision to be made after consultation if detailed cancer cell confirmation and other cancer cell line tests are desired.
- Time required: 3-4 weeks
- Gene expression (real-time PCR) and protein expression (Western blot) are determined after consultation when requested.
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Anti-inflammatory test
This is a test to compare gene and protein expression measurements of substances related to inflammation or to determine their effectiveness in inhibiting inflammation.
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- Sample type: liquid, powder, and solid
- Methods : NO assay
- Cell line: RAW264.7, decision to be made after consultation if other cells are desired
- Time required: 3-4 weeks
- Gene expression (real-time PCR) and protein expression (Western blot, ELISA) are determined after consultation when requested.
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Antioxidant test
This is a test to evaluate the antioxidant capacity of a test substance.
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- Sample type: liquid, powder, and solid
- Methods: DPPH, TPC, TFC, ORAC assay, etc.
- Time required: 3-4 weeks
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Whitening test
This is a test to measure the whitening capacity of a test substance.
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- Sample type: liquid, powder, and solid
- Methods : Tyrosinase inhibition, Melanogenesis inhibition Assay
- Cell line: SK-MEL-2, B16F10 (applied according to the test method)
- Time required: 4-10 weeks
- Gene expression (real-time PCR) and protein expression (Western blot) are determined after consultation when requested.
Development of an alternative evaluation model for animal testing
The alternative method of animal testing is a method of evaluating the efficacy and toxicity of test substances such as pharmaceuticals, cosmetics, and health functional foods without using laboratory animals, and it advances the Holosmedic HOLOSCOVERDY TM development platform by developing artificial tissues/organoids (organ analogues) such as skin, blood vessels, liver, and heart tissues.
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- 2D Cell-based Assays
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Lead Optimization
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- 3D Cell-based Assays
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- 3D Spheroid Assays
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- 3D Organoid Assays
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- 3D resistant tumor model
- Resistance process and mechanism in tumor cells
- Development of 3D resistant tumor model for research
- Human tissue model
- Development of a 3D experimental human tissue model with structure and function similar to real tissue